Rabbit Anti-NF-kB (Nuclear Factor kappa B, RelA)
The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family. Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA, LPS, UV light, and others. In most cell types, NFkB is present in the cytoplasm as a 50 kD (p50) subunit and a 65 kD (p65, rel A) subunit. In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins. Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor. In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
ELISA (Immunogen): 0.1-1ug/ml Western Blot (Human and monkey): 1-3ug/ml Immunohistochemistry (FFPE) (human): 0.5-2ug/ml. Heat-induced epitope retrieval (Citrate buffer, pH 6.0) is required Immunoprecipitation: 2-5ug/IP Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to the C-terminal portion of human NFkB. Species sequence homology: mouse and rat.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes the DNA binding subunit of human NFkB (also referred to as Rel A) at ~65kD.. Reactivity with other endogenous proteins has not been detected. Species Crossreactivity: monkey.
