Rabbit Anti-NFKB3, NT (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor Of kappa Light Polypeptide Gene Enhancer In B Cells 3, RELA)
NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human NFKB3 at N-terminal.
Form
Supplied as a liquid in 5mg BSA, 0.9mg sodium chloride, 0.2mg disodium hydrogen phosphate, 0.05mg thimerosal, 0.05mg sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NFKB3.
