N2562-08E
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IHC WBCrossreactivity
Hu Mo Rb RtGene ID
11435Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Nicotinic Acetylcholine Receptor alpha 1 (nAChR, Acetylcholine Receptor Protein alpha Chain Precursor, ACHRA, ACHRD, Cholinergic Receptor Nicotinic alpha Polypeptide 1, CHRNA1, CHNRA, CMS2A, FCCMS, SCCMS, Schizophrenia Neurophysiologic Defect Candidate)
The acetylcholine receptor of muscle, like the nicotinic acetylcholine receptor of the Torpedo electric organ, has 5 subunits of 4 different types: 2 alpha and 1 each of beta, gamma, and delta subunits. The alpha subunit exists in 2 isoforms. The protein-coding sequence of the human ACHRA gene is divided into 9 exons that correspond to different structural and functional domains of the precursor molecule. Human nicotinic acetylcholine receptor genes alpha is assigned to chromosome 2. Mutation of the acetylcholine receptor alpha subunit causes a slow-channel myasthenic syndrome by enhancing agonist binding affinity.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Immunohistochemistry (paraffin): 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to a sequence at the N-terminal of acetylcholine receptor alpha1, identical to the related mouse and rat sequences.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human nAChR. Species Crossreactivity: mouse, rat, rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Beeson, D.; Jeremiah, S.; West, L. F.; Povey, S.; Newsom-Davis, J. : Assignment of the human nicotinic acetylcholine receptor genes: the alpha and delta subunit genes to chromosome 2 and the beta subunit gene to chromosome 17. Ann. Hum. Genet. 54: 199-208, 1990. 2. Localization of the gene encoding the alpha-subunit of the acetylcholine receptor on chromosome 2 of the mouse. Cytogenet. Cell Genet. 52: 102-103, 1989. 3 Sine, S. M.; Ohno, K.; Bouzat, C.; Auerbach, A.; Milone, M.; Pruitt, J. N.; Engel, A. G. : Mutation of the acetylcholine receptor alpha subunit causes a slow-channel myasthenic syndrome by enhancing agonist binding affinity. Neuron 15: 229-239, 1995.USBio References
No references available