Technical Data

N2566-32C
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Niemann-Pick Disease, Type C1 Gene-Like 1 (NPC1L1, Niemann-Pick C1-like Protein 1, NPC1 like 1, NPC1 Niemann-Pick Disease Type C1 Gene-like 1, NPC1L1 Splice Variant, Truncated Niemann-Pick C1-like Protein 1)

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A synthetic peptide made to an internal region of rat NPC1L1 (between residues 1000-1100).
Form
As reported
Purity
Purified by immunoaffinity chromatography.
Specificity
This antibody is specific for NPC1L1 protein. Species Crossreactivity: This antibody reacts with rat and human NPC1L1 protein. There is 94% homology for mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Altmann, S.W., et al. Niemann-Pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science. 303:1201-1204, 2004. |2. Klett, E.L., & Patel, S.B. Will the real cholesterol transporter please stand up. Science. 303:1149-1150, 2004. |3. Frolov, A., et al. NPC1 and NPC2 regulate cellular cholesterol homeostasis through generation of low density lipoprotein cholesterol-derive oxysterols. J. Biol. Chem. 278(28): 25517-25525, 2003. |4. Somers, K.L., et al. Mutation analysis of feline Niemann-Pick C1 disease. Mol. Genet. Metab. 79(2): 99-103, 2003. |5. Differential trafficking of the Niemann-Pick C1 and 2 proteins highlights distinct roles in late endocyte lipid trafficking. Acta. Pediatr. Suppl. 92(443): 63-73, 2003. |6. Reid, P.C., et al. Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from Niemann-Pick type C1 mice. J. Lipid Res. 44(5): 1010-1019, 2003. |7. Altmann, S.W., et al. Niemann-Pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science. 303(5661): 1149-1150, 2004.|8. Iannou, Y.A., et al. The sructure and function of the Niemann-Pick C1 protein. Mol. Genet. Metab. 71(1-2): 175-181, 2000.|9. Identification of 58 novel mutations in Niemann-Pick disease type C: correlation with biochemical phenotype and importance of PTC1-like domains in NPC1. Hum Metab. 22(4): 313-325, 2003.
USBio References
No references available
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