Mouse Anti-NIFK (Ki-67ip, hNIFK, MKI67 FHA Domain Interacting Nucleolar Phosphoprotein, MKI67IP, NOPP34, Nucleolar Phosphoprotein Nopp34, Nucleolar Protein Interacting with the FHA Domain of pKI-67, pKi67)
NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
HeLa or Ramos
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
This antibody was developed against a synthetic peptide corresponding to a portion of human NIFK between amino acids 100-200.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
A 34kD band is observed. Species Crossreactivity: Human
