N2750-01-ML650
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™650Isotype
IgG1Clone Number
2Q2313 (FP5A)Grade
Affinity PurifiedApplications
IHC WBCrossreactivity
HuAccession #
NM_000453.1Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-NIS (Sodium Iodide Symporter) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Western Blot: Recognizes a major band at ~97kD and minor bands at 15, 30, 68 and 160kD. Immunohistochemistry: Paraffin embedded tissue sections that have been subjected to antigen retrieval with 10mM citrate buffer steam for 30 minutes. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Maltose Binding Protein fusion protein corresponding to aa468-643 of human NIS (C-terminus).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human NIS. Does recognize Maltose Binding Protein.
