N2814-50
Clone Type
MonoclonalHost
RatSource
MouseIsotype
IgG2a,kClone Number
5K31 (20d5)Grade
PurifiedApplications
FCCrossreactivity
MoShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rat Anti-NKG2A,C,E
Mouse CD94/NKG2 is a natural killer (NK) cell receptor for the non-classical MHC class I molecule Qa-1b.2, 3 NKG2 is expressed on NK cells, lymphokine-activated killer (LAK) T cells, and some CD8+ memory T cells. Two-color immunofluorescent staining with the antibody reveals two distinct NKG2+ phenotypes within the NK1.1+CD3- spleen cell population: NKG2hi (50%) and NKG2low (50%). With the exception of DBA/2J mice, NKG2 is expressed on NK1.1+ cells from all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL).1
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤1ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
CHO cells transfected with B6 allele of NKG2A gene.
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Specificity
Recognizes mouse NKG2 (Mr ~43kD). Reacts with an epitope common to the NKG2A, NKG2C and NKG2E isoforms of the CD94/NKG2 heterodimer, a member of the c-type lectin family of inhibitory receptors.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Vance, R.E. and D.H. Raulet. Personal communication. 2. Vance, R.E., J.R. Kraft, J.D. Altman, P.E. Jensen, and D.H. Raulet. 1998. J. Exp. Med. 188:1841. 3. Kraft, J.R., Vance, R.E., J. Pohl, A.M. Martin, D.H. Raulet, and Jensen, P.E. 2000. J. Exp. Med. 192:613. 4. Vance, R.E., A.M. Jamieson, and D.H. Raulet. 1999. J. Exp. Med. 190:1801.USBio References
No references available