Technical Data

N2834-25
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG1
Clone Number
ANK61
Grade
Supernatant
Applications
FC IHC IP
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-NK Cell Activation Structures (Natural Killer Cell Activation Structures)
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Frozen tissues or formalin-fixed paraffin-embedded tissues. Citric acid pretreatment is recommended for paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
IL-2-activated cultured NK cells of Wag rats
Form
Supplied as a liquid, 0.05% sodium azide.
Purity
Supernatant
Specificity
Recognizes rat NK Cell Activation Structures expressed on freshly isolated and cultured rat NK cells. Also binds to rat alpha,beta-TCR T cells and at a low level to rat B cells. Activates the lytic machinery of rat NK cells, but not of T cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Giezeman-Smits KM, Gorter A, Nagelkerke JF, Van Vlierberghe RL, van Eendenburg J, Eggermont AM, Fleuren GJ, Kuppen PJ (1997) Characterization of three new membrane structures on rat NK cells which are involved in activation of the lytic machinery. Immunobiology 197: 429-443.
USBio References
No references available
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