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N2851-75-ML650 Mouse Anti-NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C, CD161 Antigen-like Family Member C, Lymphocyte Antigen 55c, Ly-55c, NKR-P1.9, NKR-P1C, Natural Killer Cell Surface Protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c) (MaxLight 650)

Specifications
References
Brand
MaxLight™
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Conjugate
MaxLight™650
Isotype
IgG2a
Clone Number
PK136
Grade
Affinity Purified
Applications
FC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

IgG Goat anti mouse IgG: RPE (rat adsorbed)
Fusion Partners: Spleen cells from immunized (C3HxBALB/c) FI Hybrid were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Applications
Immunoprecipitation Flow Cytometry: 5-10ug/ml Complement dependent lysis: 1-10ug/ml Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Spleen and bone marrow cells from CE mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse NK1.1 cell surface antigen. Does not react with rat or human.
References
1. Koo, G.C. and Peppard, J.R. (1981). Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303.2. Koo, G.C., Dumont, F., Hackett, J.Jr., Tutt, M. and Kumar, V. (1986). The NK-1.1 (-) mouse: A model to study differentiation
USBio References
No references available
Conjugates
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