Mouse Anti-NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C, CD161 Antigen-like Family Member C, Lymphocyte Antigen 55c, Ly-55c, NKR-P1.9, NKR-P1C, Natural Killer Cell Surface Protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c) (Azide Free)
Clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161 and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
This antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
Fusion Partners: Spleen cells from immunized (C3HxBALB/c) FI Hybrid were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, and Functional Assays (in vivo depletion of NK cells, and in vitro activation of NK cells). Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 5-10ug/ml. 10ul labels 1x10e6 cells in 100ul. Functional Assays: 1-10ug/ml Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Spleen and bone marrow cells from CE mice.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the mouse NK1.1 cell surface antigen. Useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Species Crossreactivity: Does not crossreact with rat or human.
