N2915-57
Clone Type
MonoclonalHost
RabbitSource
MouseIsotype
IgGClone Number
11C1051(D98G11)Grade
PurifiedApplications
FC IC IF IHC WBCrossreactivity
Hu Mk Mo RtGene ID
10397Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-N-myc Downstream Regulated Gene 1 Protein, phosphorylated (Thr346) (NDRG1, Ndr1, Ndrl, Protein NDRG1, Protein Ndr1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, Tdd5)
NDRG1 (N-myc downstream-regulated gene 1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG1 family comprised of four members (NDRG1-4) that function in growth, differentiation and cell survival. NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage, hypoxia and elevated levels of nickel and calcium.
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, Western Blot and Immunohistochemistry . Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:800 Flow Cytometry: 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:500. Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr346 of mouse NDRG1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, and 50% glycerol.
Specificity
Recognizes mouse NDRG1, phosphorylated at Thr346. |Crossreactivity: May cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366.|Species Crossreactivity: human, monkey and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Shimono, A. et al. (1999) Mech Dev 83, 39-52. 2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9. 3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92 4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44. 5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8. 6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105. 7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40. 8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42. 9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43. 10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58. 11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56. 12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53. 13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.USBio References
No references available