Technical Data

N5375-18E-ML650
Clone Type
Monoclonal
Host
Hamster
Source
Mouse
Conjugate
MaxLight™650
Isotype
IgG
Clone Number
12G37 (HMN2-35)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Hamster Anti-NOTCH2 (Notch 2, Neurogenic Locus Notch Homolog Protein 2, hN2) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
P3U1 myeloma cells with spleen cells from Armenian hamsters.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Mouse Notch 2-Fc fusion protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse NOTCH2. Species Crossreactivity: Rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763-773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295-301. 3. Gibb, D.R. et al. (2010) ADAM10 is essential for Notch2-dependent marginal zone B cell development and CD23 cleavage in vivo. J. Exp. Med. 207: 623-635.
USBio References
No references available
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