Rabbit Anti-Nuclear Receptor Rev-ErbA alpha (Rev ErbA alpha, Rev-ErbA-alpha, Rev Erb alpha, Rev-Erb-alpha, Rev-erbalpha, Rev-ErbA, ErbA-related 1, EAR1, EAR-1, hRev, Nuclear Receptor Subfamily 1 Group D Member 1, NR1D1, Orphan Nuclear Receptor NR1D1, THRA1, Thyroid Hormone Receptor alpha-like, THRAL, V-erbA-related Protein EAR-1, V-erbA Related Protein EAR-1) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Rev-ErbA alpha (NR1D1 or nuclear receptor subfamily 1, group D, member 1), is a member of the Rev-ErbA family of nuclear receptors and is a transcriptional repressor. In mammals, Rev-Erba is highly expressed in the liver, skeletal muscle, adipose tissue, and the brain, participating in the development and circadian regulation of these tissues. Rev-Erba regulates gene transcription by directly binding to target response elements (RevREs), comprises an A/T-rich flank followed by AGGTCA. Rev-Erba mediates repression by recruiting the corepressor N-CoR, which then activates the histone deacetylase (HDAC) 3. A number of target genes has been identified for Rev-Erba, including the lipoproteins ApoA1 and ApoCIII, hydratase dehydrogenase, the circadian factor BMAL, and the anti-fibrinolytic factor PAI-1. Many of these genes are coordinately regulated by Rev-erba and the RAR-related orphan receptor RORa, which share the same response elements but exert opposite effects on gene transcription. Crosstalk between Rev-Erba and RORa likely acts to fine-tune of their target physiologic networks, such as circadian rhythms, metabolic homeostasis, and inflammation.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
A431 cell lysate
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated linear peptide corresponding to residues surrounding the area near the N-terminus region of NR1D1. Epitope: Near N-terminus. Species sequence homology: mouse and rat
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NR1D1 near the N-terminus.
