Mouse Anti-NME1 (Nucleoside Diphosphate Kinase A, NDP Kinase A, NDK A, Tumor Metastatic Process-Associated Protein, Metastasis Inhibition Factor nm23, nm23-H1, Granzyme A-Activated DNase, GAAD, NDPKA, NM23) (Azide Free) (HRP)
This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
Applications
Suitable for use in ELISA, and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 1ug/ml-3ng/ml Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length recombinant protein corresponding to aa1-153 of human NME1 with GST tag. MW of the GST tag alone is ~26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human NME1.
