O5202-ML550
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™550Isotype
IgG1,kClone Number
3G217Grade
Affinity PurifiedApplications
FLISA IC IF WBCrossreactivity
Ca Hu Ma Mo RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Occludin (MaxLight 550)
Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1).
Applications
Suitable for use in FLISA, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Positive Control
T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma).
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
GST fusion protein consisting of the C-terminal region (~150aa) of human occludin. Species Sequence Homology: Bacteria, C.elegans, fish, guinea pig
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Occludin Protein. Species Crossreactivity: mouse, rat and canine. Does not react with sheep, porcine, rabbit, rhesus monkey
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Anderson, J.M., et al., Curr. Opin. Cell. Biol. 5: 772-778 (1993). 2. Gumbiner, B., Am. J. Physiol. 123: 1631-1633 (1993). 3. Farquhar, M., and Palade, G., J. Cell. Biol. 17: 375-409 (1963). 4. Stevenson, B.R., et al., J. Cell. Biol. 103: 755-766 (1986). 5. Anderson, J.M. et al. (1988) J. Cell. Biol. 106:141-1149. 6. Itoh, M., et al., J. Cell. Biol. 115: 1449-1462 (1993). 7. Gumbiner, B., et al., PNAS USA 88: 3460-3464 (1991). 8. Citi, S., et al., Nature 33: 272-276 (1988). 9. Zhong, Y., et al., J. Cell. Biol. 120: 477-483 (1993). 10. Keno, B.H., et al., J. Cell. Biol. 134: 1003-1018 (1996). 11. Furuse, M., et al., J. Cell Biol. 123: 1777-1788 (1993). 12. Furuse, M., et al., J. Cell. Biol. 127: 1617-1626 (1994). 13. Ando-Akatsuka, Y., et al., J. Cell. Biol. 133: 43-47 (1996).USBio References
No references available