Goat Anti-Pou5f1 (POU Domain, Class 5, Transcription Factor 1, NF-A3, Octamer-binding Protein 3, Oct-3, Octamer-binding Protein 4, Oct-4, Octamer-binding Transcription Factor 3, OTF-3, Oct-3, Oct-4, Otf-3, Otf3)
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Western Blot: 1-3ug/ml, observed in lysates of cell line NIH3T3 on ~40kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DRPNAVKLEKVEP, from mouse OCT4, at the internal region of the protein (NP_038661.2). Species sequence homology: Rat.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse OCT4.
