O7700-01
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
Hu MoShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)Rabbit Anti-ORAI2 (CAP-Binding Protein Complex-Interacting Protein 2)
Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels (1 for review). ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC (2). Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor (3,4), although at a lesser efficacy than ORAI1 (4,5). This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.
Applications
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2 ug/ml. Immunocytochemistry: 5ug/ml. Optimal dilutions to be determined by the researcher.
Positive Control
Jurkat cell lysate
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
15 amino acid peptide from near the carboxy terminus of human|ORAI2 (UniProt # Q96SN7).
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ORAI2. Does not cross react with ORAI1 or ORAI3. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. 2. Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85. 3. Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in interorganelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8. 4. Mercer JC, DeHaven WI, Smyth JT, et al. Large storeoperated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90. 5. DeHaven WI, Smyth JT, Byles RR, et al. Calcium inhibition and calcium potentiation of Orai1, Orai2 and Orai3 calciumrelease-activated calcium channels. J. Biol. Chem. 2007;USBio References
No references available