Rabbit Anti-Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like)
Periostin is a disulfide linked 90kD, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP-2). OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.
Applications
Suitable for use in ELISA, Western Blot, Immmunofluorescence, Indirect Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE): 1:100. Heat induced antigen retrieval with EDTA Buffer, pH 9.0 is recommended. Immunofluorescence: 1:10-1:50 Flow Cytometry (Indirect): 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 687-716 in the C-terminal region of human Osteoblast Specific Factor 2.
Form
Supplied as liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human Osteoblast Specific Factor 2. Species Crossreactivity: rat
