Suitable for use in RIA and ELISA. Other applications not tested.

RIA: 1:2000 ELISA: 1:5000 Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide corresponding to aa1-22 of human Osteocalcin (MRALTLLALLALAALCIAGQAG).

Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.

Serum

Recognizes human osteocalcin (1-22) mature form, osteocalcin |(1-44) and osteocalcin (1-49). No reactivity with human bone sialoprotein, human osteopontin and human osteonectin.