Technical Data

O8075-35
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Isotype
IgG1,k
Clone Number
6F162 (6C8)
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Ovalbumin, Denatured

Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. Native chicken ovalbumin is a globular monomer with a molecular mass of 45 kDa. It is structurally related to both serpins and lipocalins.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Ovalbumin isolated from chicken egg white, heat-denatured.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography from culture supernatant.
Specificity
Specific for denatured chicken OA (2). Cross-reactivity to egg albumines from other species has not been tested. Epitope specificity differs from that of clones 6F161 and 6F163, as determined by inhibition ELISA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Nisbet AD, Saundry RH, Moir AJ, Fothergill LA, Fothergill JE (1981) The complete amino acid sequence of hen ovalbumin. Eur J Biochem 115:335-345. 2. Koch C, Jensen SS, Oster A, Houen G (1996) A comparison of the immunogenicity of the native and denatured forms of a protein. APMIS 104:115-125. 3. Houen G, Koch C (1997) A non-denaturing enzyme linked immunosorbent assay with protein preadsorbed onto aluminum hydroxide. J Immunol Methods 200:99-105.
USBio References
No references available
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