O8233-50
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IC WBCrossreactivity
Hu Mk Mo Po RtShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-OxoETE Receptor, 5- (TG1019, R527, OXER1)
5-Oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-OxoETE) is a potent stimulator of chemotaxis for eosinophils and neutrophils that mediates its action via a putative G-protein coupled receptor (GPCR). The 5-OxoETE receptor couples to Gi/o to inhibit cyclic AMP production and to mobilize intracellular calcium. Northern blot analysis reveals that the receptor is highly expressed on eosinophils and neutrophils, suggesting a key role in the inflammatory response. In addition, the protein can also be detected in tissues such as kidney and liver.
Applications
Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:200 Immunocytochemistry: 1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the C-terminal region of human 5-OxoETE receptor.
Form
Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human 5-OxoETE receptor. Species Crossreactivity: African green monkey, mouse, porcine and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Hosoi, T., Koguchi, Y., Sugikawa, E., et al. Identification of a novel human eicosanoid receptor coupled to Gi/o. J. Biol. Chem. 277(35), 31459-31465 (2002).|2. Jones, C.E., Holden, S., Tenaillon, L., et al. Expression and characterization of a 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid receptor highly expressed on human eosinophils and neutrophils. Mol. Pharmacol. 63(3), 471-477 (2003).USBio References
No references available