O8250-01A
Clone Type
PolyclonalHost
RabbitIsotype
IgGGrade
SerumApplications
IHC RIA WBCrossreactivity
Bo HuGene ID
280888Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Oxytocin (OXT, OT NPI, Oxytocin Neurophysin Preproprotein, Oxytocin Preproprotein,|Oxytocin Prepro (Neurophysin I)) (Not for Export EU)
Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behavior, as well as in the regulation of water excretion and cardiovascular functions.
Applications
Suitable for use in RIA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
RIA: 1:80,000 Immunohistochemistry (Paraffin): 1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic Oxytocin, conjugated to bovine thyroglobulin
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O.
Specificity
Recognizes Oxytocin. Does not crossreact with Lys-/Arg-vasopressin, neurophysin or pituitary hormones (e.g. prolactin). Species Crossreactivity: human and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Schams D, Schmidt-Polex B, Kruse V. Oxytocin determination by radioimmunoassay in cattle. I. Method and preliminary physiological data Acta Endocrinol (Copenh). 1979 Oct; 92(2):258-270. 2. Schams D, Lahlou-Kassi A, Glatzel P. Oxytocin concentrations in peripheral blood during the oestrous cycle and after ovariectomy in two breeds of sheep with low and high fecundity. J Endocrinol. 1982; 92(1):9-13. 3. Schams D. Oxytocin determination by radioimmunoassay. III. Improvement to subpicogram sensitivity and application to blood levels in cyclic cattle, Acta Endocrinol (Copenh). 1983 Jun;103(2):180-183. 4. Walters DL, Schams D, Schallenberger E.. Pulsatile secretion of gonadotrophins, ovarian steroids and ovarian oxytocin during the luteal phase of the oestrous cycle in the cow. J Reprod Fertil. 1984; 71(2):479-491. 5. Kruip TA, Vullings HG, Schams D, Jonis J, Klarenbeek A Immunocytochemical demonstration of oxytocin in bovine ovarian tissues, Acta Endocrinol (Copenh). 1985 Aug;109(4):537-542.USBio References
No references available