Mouse Anti-p19 (CDKN2D, Cyclin-dependent kinase 4 inhibitor D, p19-INK4d)
Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclindependent kinases. The activity of the cyclin D-dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d. The p19 protein, also known as INK4d was first identified in a yeast two-hybrid system screened for CDK4 binding proteins. Human p19 shares 86% identity at the amino acid level with murine p19, and is ~44% identical to each of the other human INK4 family members.
Applications
Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant protein corresponding to human p19.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes human p19. Identifies a band at ~19kD on Western Blots.
