Technical Data

P1001-05U
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_004055
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-p27Kip1, CT (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)

p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human 27Kip1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human 27Kip1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005). 2. Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005). 3. Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). 4. Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). 5. Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
USBio References
No references available
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