Rabbit Anti-p27Kip1 (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, CDKN1B, KIP1)
Cyclin-dependent kinase inhibitor 1B (CDKN1B), also known as p27 (KIP1), is a cyclin-dependent kinase (Cdk) inhibitor implicated in G1 phase arrest, which negatively regulates G1 phase progression in response to TGF beta and represents a tumor suppressor gene. Human p27 gene is mapped to chromosome 12p12.3 p27 can be both an inhibitor and a substrate of cyclin E-CDK2. p27, abundant in quiescent cells and drops after serum stimulation, plays a role in mediating VSMC hypertrophy. p27 degradation is subject to dual control by the accumulation of both SKP2 and cyclins following mitogenic stimulation. It regulates cell proliferation by binding to and modulating the activity of cyclin-dependent kinases. Reduced p27 activity is fundamental for the development of many human malignancies including breast, prostate, colon and gastric carcinomas.
Applications
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot:1-2ug/ml Immunohistochemistry (paraffin):1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peptide mapping at the C-terminal end of P27 of human origin, differs from the related rat sequence by single aa.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human p27Kip1. Species Cross-Reactivity: mouse, rat.
