Mouse Anti-p53 Binding Protein 1 (53BP1, 53-BP1, FLJ41424, MGC138366, p202, p53BP1, Tumor Protein 53 Binding Protein 1, Tumor Protein p53 Binding Protein 1, TP53BP1, Tumor Suppressor p53 Binding Protein 1)
53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. Small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrated that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-4ug/ml detected 53BP1 in lysates from HeLa cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate 53BP1 from HeLa cell lysate. Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for 53BP1 in HeLa cells fixed with 3% paraformaldehyde and permeabilized in 0.5% Triton X-100. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1. UniProt Accession: Q12888
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human 53BP1, Mr ~250kD. Does not react with mouse.
