Mouse Anti-p53, aa181-190 (Tumor Suppressor Protein, Oncogene Protein) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
Recommended Positive Control Tissue: Colon or breast carcinoma
Recommended Negative Controls: Mouse IgM Negative Control
Applications
Immunohistochemistry: Frozen, Paraffin. 1:1000 Western Blotting: 1:1000 Immunoprecipitation Optimal working dilutions to be determined by researcher.
Immunohistochemistry: *This product does require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human p53.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the human p53 tumor suppressor protein, binding to both wild type and mutant forms. 3H2809 recognizes an epitope within the central region of p53 between amino acids 181-190.
