Technical Data

P1001-32D
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
9E11/E11
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-p53 (Tumor Suppressor Protein, Oncogene Protein)

The C terminus of p53 is rich in lysines, which are subjected to different modifications to regulate p53 activity. p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas. Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene. The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression. p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
KLH-conjugated synthetic peptide SHLKSKKGQST corresponding to aa367-377 of human tumor suppressor p53
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human p53

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Chuikov, S. et .al. (2004) Nature 432,353 �" 360 ; Hupp, T.R. et. al. (1992) Cell 71,875 �" 886 ; Malkin, D. et. al. (1990) Science 250,1233 �" 1238.
USBio References
No references available
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