Technical Data

P1010-02B
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
RW105
Grade
Affinity Purified
Applications
IF IP WB
Crossreactivity
Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-p300 (CREBBP/EP300 Inhibitory Protein 1, Cyclic AMP Responsive Enhancer Binding Protein, EP300: E1A Binding Protein p300, EP300, RB and P300 Binding Protein EID 1, Retinoblastoma Protein Associated Protein)

p300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which is conserved among a variety of mammalian species. It is actively synthesized and phosphorylated in both quiescent and proliferating cells.

Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators and have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes. As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.
Cellular Localization: Nuclear
Applications
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Fusion protein containing residues 1572-2371 of human p300.
Form
PBS [pH7.4] containing 0.09% sodium azide
Purity
Purified by Protein G affinity chromatography.
Specificity
This antibody is specific for p300 protein. This antibody recognizes residues 1921-2023. Species Crossreactivity: This antibody reacts with human, mouse, rat, monkey, and mink protein. Other species have not been tested.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Eckner, R., et al. Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor. Genes & Dev., 8: 869-884 (1994)|2. Eckner, R., et al. Association of p300 and CBP with simian virus 40 large T antigen. Mol. & Cell Bio., 16: 3454-3464 (1996)|3. Eckner, R., et al. Interaction and functional collaboration of p300-CBP anf bHLH proteins in muscle and B-cell differentiation. Genes & Dev., 10: 2478-2490 (1996)
USBio References
No references available
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