Technical Data

P1792-90
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Bo Ca Eq Hu Mo
Accession #
NP_060496
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PACS1 (Phosphofurin Acidic Cluster Sorting Protein 1, PACS-1, KIAA1175)

PACS-1 (CRA_a) is a 963aa rich regulatory protein involved in protein trafficking and belongs to the PACS family. A coat protein involved in controlling the correct subcellular localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs, PACS-1 is also known to be involved in HIV-1 Nef-mediated removal of MHC-I from the cell surface to the TGN, thus enabling HIV-1 to escape immune surveillance. PACS-1 also controls the endosome to Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin coated membrane pits. Usually seen in golgi apparatus, trans-Golgi network and localized in the paranuclear region, it is ubiquitously expressed in most of the tissues.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.05-0.1ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Human uterus lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa300-350 of human PACS-1.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PACS-1. Species Crossreactivity: chimpanzee, bovine, canine, equine, mouse, opossum. Species sequence homology: chicken, zebrafish.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Blagoveshchenskaya, AD. et al. Cell. 111:853-866 (2002). 2. Kottgen, M. et al. EMBO J. 24:705-716 (2005). 3. Simmen, T. et al. EMBO J. 24:717-729 (2005). 4. Wan, L. et al. Cell. 94:205-216 (1998).
USBio References
No references available
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