Rabbit Anti-PACSIN2 (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 2)
PACSIN2, a 486aa phosphoprotein is a novel member of the PACSIN family of cytoplasmic adapter proteins. It is an isoform of PACSIN with a CDC15 N-terminal domain, a C-terminal SH3 domain, 3 conserved regions specific to the PACSIN family, and 3 asn-pro-phe (NPF) motifs, which potentially bind to EH domains. This protein is known to display a ubiquitous expression pattern with the highest levels of expression in brain. Reports suggest that PACSIN2 participates in the organization of the actin cytoskeleton, the regulation of vesicular traffic. It also participates in endocytic internalization and endocytic receptor recycling.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.02-1ug/ml Immunohistochemistry (paraffin): 10ug/ml. Heat induced antigen retrieval in citrate buffer, pH 6.0 is required. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa400-450 of human PACSIN2.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PACSIN2.
