Technical Data

P1794-57E
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_036519
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PAD4, CT (Protein-arginine Deiminase Type IV, Protein-arginine Deiminase Type-4, Peptidylarginine Deiminase IV, HL-60 PAD, PADI4, PADI5, PDI5)

PAD4 is a member of a family of enzymes responsible for the conversion of arginine residues to citrulline residues via catalyzation of the deimination of the arginine residues. PAD4 down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. This protein may play a role in granulocyte and macrophage development leading to inflammation and immune response.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human PAD4 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human PAD4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Nakayama-Hamada, M., et al., Biochem. Biophys. Res. Commun. 327(1):192-200 (2005). 2.Wang, Y., et al., Science 306(5694):279-283 (2004). 3.Arita, K., et al., Arthritis Rheum. 11(8):777-783 (2004). 4.Barton, A., et al., Arthritis Rheum. 50(4):1117-1121 (2004). 5.Suzuki, A., et al., Nat. Genet. 34(4):395-402 (2003).
USBio References
No references available
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