P2488-27B
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E IHC IP WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-PAK5 (p21 Activated Kinase 5, p21-activated Kinase 5, PAK5, PAK-5, KIAA1264, MGC26232, p21(CDKN1A)-activated Kinase 7, p21-activated Kinase 7, PAK7, PAK-7, Protein Kinase PAK5, Serine/threonine-protein Kinase PAK 7)
EC=2.7.11.1, p21 Protein (Cdc42/Rac)-activated Kinase 7
PAK5 is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression.
Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:10-1:50 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from corresponding to a portion of amino acids within aa183-198 of human PAK5 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PAK5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Jaffer, Z.M., et al., Int. J. Biochem. Cell Biol. 34(7):713-717 (2002). 2.Pandey, A., et al., Oncogene 21(24):3939-3948 (2002). 3.Dan, C., et al., Mol. Cell. Biol. 22(2):567-577 (2002). 4.Cau, J., et al., J. Cell Biol. 155(6):1029-1042 (2001).USBio References
No references available