P3105-60-APC
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
APCIsotype
IgG1Clone Number
3G232Grade
Affinity PurifiedApplications
FC FLISA IC IFCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
BSA Free
Mouse Anti-PAR1 (Proteinase Activated Receptor1) (APC)
Serine proteases comprise a large family of enzymes that are characterized by a uniquely reactive Ser side chain. They are ubiquitous in prokaryotes and eukaryotes and serve important and diverse biological functions. Although serine proteases are usually considered to act principally as degradative enzymes, certain proteases are signaling molecules that specifically regulate cells by cleaving and triggering members of a new family of Proteinase Activated Receptors (PARs). There are three members of this family, PAR-1 and PAR-3, which are receptors for thrombin, and PAR-2, a receptor for trypsin and mast cell tryptase. Proteases cleave within the extracellular NH2-terminus of their receptors to expose a new NH2-terminus. In common with many G protein-coupled receptors, PARs couple to multiple G proteins and thereby activate many parallel mechanisms of signal transduction. PARs are expressed in multiple tissues by a wide variety of cells, where they are involved on several pathophysiological processes, including growth and development, mitogenesis, and inflammation.
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry and FLISA. Not recommended for use in Western Blot. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not crossreact with PAR2. Has been shown to completely inhibit the cleavage and activation of PAR1 by thrombin when used in combination with a second PAR1-directed antibody.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Dery, O., et al., AJP-Cell Physiology 274(6): C1429 (1998). 2. O’Brien, P.J., et al., J. Biol. Chem. 275(18): 13,502–13,509 (2000). 3. Zacharias, U., et al., Exp. Cell. Res. 216(2): 371–379 (1995). 4. Weinstein, J.R., et al., J. Neurochem. 71(13): 1034–1050 (1998). 5. Xu, Y., et al., Am. J. Pathol. 146(1): 101–110 (1995). 6. Andrade-Gordon, P., et al., Proc. Natl. Acad. Sci. 96(22): 12,257–12,262 (1999).USBio References
No references available