Rabbit Anti-PARP (Poly ADP-Ribose Polymerase)
PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates PARP's amino-terminal DNA binding domain (24kD) from its carboxy-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Applications
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (IC): 1:800 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Gly623 of human PARP.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of total full-length human PARP at ~116kD and the large fragment (89kD) produced by caspase cleavage. Does not react with PARP2 or PARP3. Species Crossreactivity: monkey, mouse and rat
