Mouse Anti-PARP, cleaved (Poly ADP-Ribose Polymerase, ADP Ribosyltransferase NAD+, ADPRT, ADPRT1, NAD(+) ADP Ribosyltransferase 1, pADPRT-1, Poly Adenosine Diphosphate ADP Ribose Polymerase, Poly [ADP-ribose] Polymerase 1, PARP1, PARP-1, Poly (ADP-ribose) Polymerase Family Member 1, Poly [ADP-ribose] Synthetase 1, PPOL)
The poly (ADP-ribose) polymerase (PARP) is involved in cell recovery from DNA damage, such as methylation of N3-adenine, which activates the base excision repair process. PARP [Poly (ADP-ribose) polymerase] is a 116kD nuclear chromatin-associated enzyme that is cleaved during apoptosis by caspase-3 into a 24kD fragment containing the DNA binding domain and an 89kD fragment containing the catalytic and automodification domains. The 24kD-fragment irreversibly bind to DNA and may contribute to the irreversibility of apoptosis by blocking the access of DNA repair enzymes to DNA strand breaks.
Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1-2ug/ml Flow Cytometry (intracellular): 1-10ug/1x10e6 cells Optimal dilutions to be determined by the researcher.
Positive Control
Camptothecin treated HL-60 (12 hr)
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to amino acids near 214/215-cleavage site of human PARP.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes a human 89kD cleavage fragment containing the catalytic and auto modification domain. Reported to react with mouse but not to react with rat.
