Technical Data

P3113-07
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Mk Mo Rt
Gene ID
142
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-PARP (Poly ADP-Ribose Polymerase)

PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARP’s N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the caspase cleavage site in PARP.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of full length human PARP (116kD), as well as the large fragment (89kD) and small fragment (24kD) of PARP resulting from caspase cleavage. Does not crossreact with related proteins. Species Crossreactivity: mouse, monkey and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Satoh, M.S. & Lindahl, T., Nature 356: 356-358 (1992). 2. Lazebnik, Y.A., et al., Nature 371: 346-347 (1994). 3. Cohen, G.M., Biochem. J. 326: 1-16 (1997). 4. Nicholson, D.W., et al., Nature 376: 37-43 (1995). 5. Tewari, M., et al., Cell 81: 801-809 (1995). 6. Oliver, F.J., et al., J. Biol. Chem. 273: 33,533-33,539 (1998). 7. Li, J., et al., J. Biol. Chem. 277: 388-394 (2002). 8. Soldatenkov, V.A., et al., J. Biol. Chem. 277: 665-670 (2002).
USBio References
No references available
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