Rabbit Anti-PARP 1, cleaved (Asp214) (Poly ADP-Ribose Polymerase 1, Poly [ADP-ribose] Polymerase 1, PARP1, PARP-1, PARP, ADPRT 1, ADPRT1, ADPRT, NAD(+) ADP-ribosyltransferase 1, pADPRT-1, Poly[ADP-ribose] Synthase 1, PPOL)
Poly (ADP-ribose) Polymerase 1, EC=2.4.2.30
PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24kD) from the carboxy-terminal catalytic domain (89kD) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunofluorescence (IC): 1:400 Flow Cytometry (Fixed/Permeabilized): 1:200-1:800 Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (paraffin): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Asp214 in human PARP.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity
Recognizes endogenous levels of the large fragment (89kD) of human PARP1. Does not recognize full length PARP1 or other PARP isoforms. Species Crossreactivity: monkey.
