P3113-82E
Clone Type
MonoclonalHost
RabbitSource
HumanIsotype
IgGClone Number
7H49(C53A3)Grade
PurifiedApplications
IP WBCrossreactivity
HuGene ID
5727Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Patched 1 (PTCH1)
Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methyl-glutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10). PTCH1 itself is a target of Hedgehog signaling (11), with elevated PTCH1 expression as a surrogate marker for Hedgehog pathway activation (12-14).
Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues surrounding Pro1307 of human PTCH1.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity
Recognizes transfected levels of human PTCH1. Also recognizes endogenous levels of PTCH1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Benameur, T. et al., (2010) PLoS ONE 5: e12688. doi:10.1371/journal.pone.0012688. 1. Stone, D.M. et al. (1996) Nature 384:129-134. 2. Chen, Y. et al. (1996) Cell 87:553-563. 3. Motoyama, J. et al. (1998) Nat. Genet. 18:104-106.4. Smyth, I. et al. (1999) Hum. Mol. Genet. 8:291-297. 5. Ingham, P.W. et al. (2001) Genes Dev. 15:3059-3087. 6. McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53:1-114. 7. Hahn, H. et al. (1996) Cell 85, 841-851. 8. Johnson, R.L. et al. (1996) Science 272, 1668-1671. 9. Kuwabara, P.E. et al. (2002) Trends Genet. 18:193-201. 10. Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22:129-157. 11. Agren, M. et al. (2004) Gene 330:101-114. 12. Watkins, D.N. et al. (2003) Nature 422:313-317. 13. Berman, D.M. et al. (2003) Nature 425:846-851. 14. Karhadkar, S.S. et al. (2004) Nature 431:707-712.USBio References
US Biological application reference: Benameur, T. et al., (2010) PLoS ONE 5: e12688. doi:10.1371/journal.pone.0012688.