Mouse Anti-Pax5 (Paired Box 5, Paired Box Homeotic Gene 5, Pax-5, B cell Lineage Specific Activator Protein, B cell Specific Transcription Factor, BSAP)
Paired box protein Pax-5 is a member of the paired box (PAX) family of transcription factors. This family has a highly conserved DNA-binding motif known as the paired box. Paired box protein Pax-5 may play an important role in B-cell differentiation as well as neural development and spermatogenesis. It is involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. It localises to the nucleus and is expressed at early B-cell differentiation in the developing CNA and in adult testis. A defect in Paired box protein Pax-5 is a cause of acute lymphoblastic leukemia.
Applications
Suitable for use in Western Blot, Immunohistochemistry, and ELISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Partial recombinant protein corresponnding to aa192-302 of human Paired box protein Pax-5 with a GST tag.
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Pax5.
