Technical Data

P3113-90Y
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Paxillin, phosphorylated, Tyr31 (PXN)

Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Tyrosine 31, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control
NMuMG cells
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human paxillin that contains tyrosine 31.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse paxillin when phosphorylated at Tyr31. Species sequence homology: Human (100%) and chicken (93%).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Grace, E.A. and J. Busciglio (2003) Aberrant activation of focal adhesion proteins mediates fibrillar amyloid beta-induced neuronal dystrophy. J. Neurosci. 23(2):493-502. 2. Levkau, B., et al. (2002) Activation of metalloproteinases and their association with integrins: an auxiliary apoptotic pathway in human endothelial cells. Cell Death Differ. 9(12):1360-1367 (cites the use of this antibody). 3. Manabe, Ri R., et al. (2002) GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration. J. Cell Sci. 115(Pt 7):1497-1510. 4. ,Melendez, J., et al. (2002) Activation of Pyk2/Related Focal Adhesion Tyrosine Kinase and Focal Adhesion Kinase in cardiac remodeling. J. Biol. Chem. 277(47):45203-45210. 5. Ushio-Fukai, M., et al. (2001) Cholesterol detection inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells - Role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling. J. Biol. Chem. 276(51):48269-48275. 6. Weinberg, J.M., et al. (2001) Energetic determinants of tyrosine phosphorylation of focal adhesion proteins during hypoxia/reoxygenation of kidney proximal tubules. Am. J. Pathol. 158(6):2153-2164. 7. Sussman, M.A., et al. (2000) Altered focal adhesion regulation correlates with cardiomyopathy in mice expressing constitutively active rac1. J. Clin. Invest. 105(7):875-886. 8. Nakamura, K., et al. (2000) Tyrosine phosphorylation of paxillin a is involved in temporospatial regulation of paxillin-containing focal adhesion formation and F-actin organization in motile cells. J. Biol. Chem. 275(35):27155-27164. 9. Schaller, M.D. and J.T. Parsons (1995) pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk. Mol. Cell. Biol. 15(5):2635-2645.
USBio References
No references available
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