Rabbit Anti-Pax 2, phosphorylated (Paired Box Gene 2, Paired Box Homeotic Gene 2, Paired Box Protein 2, Paired Box Protein Pax-2, Paired Box Protein Pax2, Pax-2)
Pax2 is a transcription factor encoded by a gene that is required for the embryonic development of a variety of mammalian tissues, including the kidney, optic cup, and inner ear. Structurally, the Pax2/5/8 protein subfamily is characterized by an N-terminal paired domain, a conserved octapeptide sequence, and a paired-paired type homeobox. The N-terminal paired domain of the Pax2/5/8 proteins binds DNA, while the C-terminal region is required for activation of target genes. In human mammary tissue, Pax2 expression has been identified in sub-populations of mammary ductal cells, and also in > 50% of human breast tumors surveyed (n=38). Mouse studies have demonstrated co-localization of Pax2 and WT1 (Wilms’ tumor suppressor protein) in the nuclei of normal and cancerous breast cells, indicating a role for Pax2 in the regulation of the response to progesterone in the mature mammary gland. Pax2 is a phosphorylation target of JNK, and has been observed to co-imunoprecipitate with JIP1. Phosphorylation of Pax2 enhances its ability to transactivate reporter genes.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml; Western Blot analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Species Sequence Homology: mouse, chicken, zebrafish and frog; 100%.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Pax2 protein when phosphorylated at Ser394 in the 416aa isoform and at Ser371 of the 393 isoform. |Sequence homology: Pax5 and Pax8. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK.
