Technical Data

P3115-11A
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG2a
Clone Number
2Q483 (PC10)
Grade
Purified
Applications
FC IHC IP WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-PCNA (Proliferating Cell Nuclear Antigen)

Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

Applications
Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.1-1ug/ml Flow Cytometry: 1:100 Immunohistochemistry (formalin fixed paraffin embedded sections only): 5-50ug/ml. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin's reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended. Optimal dilutions to be determined by the researcher.
Positive Control
Tonsil or reactive lymph node.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat PCNA made in the protein A vector pR1T2T.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes rat PCNA. Species Crossreactivity: All vertebrate species and insects.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Wassem, N.H. and Lane, D.P., J. Cell Science 96:121–129 (1990). 2. Hall, P.A., et al., J. Pathology 162:285–294 (1990). 3. Coltrera, M.D. and Gowan, A.M., J. Histochemistry and Cytochemistry 39:23–30 (1991). 4. Takasaki, Y., et al., J. Exp. Medicine 154:1899–1909 (1981).
USBio References
No references available
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