P3115-14
Clone Type
MonoclonalHost
MouseSource
RatIsotype
IgG2aClone Number
PC10Grade
PurifiedApplications
IC IF IHC WBCrossreactivity
Hu RtShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-PCNA (Proliferating Cell Nuclear Antigen)
PCNA, originally known as cyclin, is a 36kD nuclear protein, which plays an integral role in the eukaryotic cell cycle. PCNA is essential for cellular DNA synthesis, and it is also required for the in vitro replication of simian virus 40 (SV 40). PCNA was recently identified as an auxiliary protein of DNA polymerase-d and acts to coordinate leading and lagging strand synthesis at the replication fork. It has been suggested that expression of high levels of PCNA protein correlates with organ metastasis and vascular invasion. Therefore, detection of high levels of PCNA may be an effective prognostic indicator. The mouse anti-PCNA antibody is a useful tool for studying the proliferating cells in normal tissues, and possible aberrant expression in neoplasms.
Applications
Suitable for use in Immunofluorescence, Western Blotting, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blotting: 0.25 – 1.0ug/ml Immunohistochemistry: 5ug/ml (Alcohol or formalin-fixed, paraffin-embedded) Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat recombinant PCNA polypeptides
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity
Purified from ascites raised in Balb/C mice.
Specificity
Reacts with PCNA (36kD) in human, primate, insect and yeast., S.pombe (1)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Waseem, NH and Lane, DP; J Cell Sci 96:121 (1990). 2. Maeda, MD, et al; Cancer 73:528–533 (1994). 3. Cardoso, C, et al; Cell 74:979–992 (1993). 4. Hall, PA, et al; J Pathol 162:285–294 (1990). 5. Robbins, B, et al; Arch Pathol Lab Med 111:841 (1987). 6. Medrano, E.E., et al; Cancer Research 55:4047–4052 (1995). 7. Pe’er, J. et al; Opthamology 101 (1): 56–62 (1994). 8. Huang, G.Y., et al; J. Cell Biol. 143(6): 1725–1734 (1998). 9. Shpitz, B., et al; Human Pathology 29(5): 469–475 (1998). 10. Schneider, J. et al; Science 264:1467 (1994).USBio References
No references available