Technical Data

P3120-63
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG1,k
Clone Number
9A191 (MIH42)
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-CD276 (B7-H3) (PE)
B7RP2

B7-H3, assigned as CD276, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human B7-H3 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain, a signature intracellular domain, and an additional isoform, known as 4Ig-B7-H3, containing nearly exact tandem duplication of the IgV-IgC domain and most likely caused by exon duplication. B7-H3 mRNA is broadly expressed in normal tissues whereas its protein expression is relatively rare. The expression of B7-H3 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages. It can be upregulated during the maturation from monocytes to DCs, or during the interaction between DCs and regulatory T cells. B7-H3 has been shown to be a co-stimulatory molecule that inhibits T-cell responses. B7-H3 has also been identified to bind TLT-2 involved in the intracellular signaling pathway.

Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 5ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Human B7-H3
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD276. Species Crossreactivity: African Green, Cynomolgus, Rhesus

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Chapoval, A. I. et al. (2001) B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat. Immunol. 2: 269-274. 2. Sun, X. et al. (2003) Mouse B7-H3 induces antitumor immunity. Gene Ther. 10: 1728-1734. 3. Ma, L. et al. (2007) Complete eradication of hepatocellular carcinomas by combined vasostatin gene therapy and B7H3-mediated immunotherapy. J. Hepatol. 46: 98-106.
USBio References
No references available
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