Goat Anti-PDLIM1 (PDZ and LIM Domain Protein 1, C-terminal LIM Domain Protein 1, Elfin, LIM Domain Protein CLP-36, CLIM1, CLP36)
Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 0.1-0.3ug/ml, observed in human lung and heart lysates on ~37kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-TPPEGYEVVTVFPK, from human PDLIM1, at C-terminal (NP_066272.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PDLIM1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Kotaka M, Kostin S, Ngai S, Chan K, Lau Y, Lee SM, Li H, Ng EK, Schaper J, Tsui SK, Fung K, Lee C, Waye MM. Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2. J Cell Biochem. 2000 Jun 12;78(4):558-65.
USBio References
No references available
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