Technical Data

P3139-86
Clone Type
Polyclonal
Host
Sheep
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Sheep Anti-PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes)

PEA-15 (Phosphoprotein enriched in astrocytes of 15kD) is a 15kD cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130aa in length. It contains a death effector domain (aa3-81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to FADD, blocking apoptosis. And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation. There is one potential alternate start site 21aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36-58. Full-length human and mouse PEA-15 show 99% aa identity.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human PEA-15 aa 2-130; Q15121 (E. Coli).
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human and mouse PEA-15.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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