Technical Data

P3240-04N
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PEPCK2, NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial), PEPCK-2, PEPCK, PCK2, Mitochondrial Phosphoenolpyruvate Carboxykinase 2, Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial, PEPCK-M, PEP Carboxykinase, Phosphoenolpyruvate Carboxylase)
EC=4.1.1.32, GTP Mitochondrial Precursor, HGNC:8725

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-terminal region of human PCK2 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PCK2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2.Modaressi, S., et al., Biochem. J. 333(Pt 2), 359-366 (1998). 3.Modaressi, S., et al., Biochem. J. 315(Pt 3), 807-814 (1996).
USBio References
No references available
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