Technical Data

P3350-27
Clone Type
Polyclonal
Host
Sheep
Isotype
IgG
Grade
Serum
Applications
DB E IP WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Sheep Anti-Peroxidase, Horseradish (HRP) (Not for Export EU)

Horseradish Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes. Horseradish Peroxidase has been found well suited for the preparation of enzyme conjugated antibodies, due in part to its ability to yield chromogenic products and to its relatively good stability characteristics.

Applications
Suitable for use in ELISA, Dot Blot, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
ELISA: 1:10,000-1:40,000 Western Blot: 1:1000-1:4000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peroxidase (Horseradish).
Form
Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 2ml sterile ddH20
Purity
Serum
Specificity
Recognizes horseradish peroxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase (Horseradish).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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