P3353-01M
Clone Type
PolyclonalHost
GoatSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
Ca Hu Mo RtAccession #
NP_071404.2Gene ID
64065, 64058, 292949Shipping Temp
Blue IceStorage Temp
-20°CGoat Anti-PERP (p53 Apoptosis Effector Related to PMP-22, Keratinocyte-associated Protein 1, KCP1, KCP-1, KRTCAP1, P53-induced Protein PIGPC1, PIGPC1, Transmembrane Protein THW, THW)
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64,000 Western Blot: 1-3ug/ml, observed in human liver lysates on ~20kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to CLPNYEDDLLGNAK, from human PERP, at C-terminal (NP_071404.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PERP. Species sequence homology: Canine, mouse and rat.
